Abstract
Authors
Long Qian, Megan L. Baker, Laura Aponte Becerra, Cathleen Liang, Emma Koval, Kyra Shelton, Daris Javed, Gilbert Moeckel, Deepika Kumar, Avi Z. Rosenberg, Michael Kuperman, Tinyi Chu, Wassim Obeid, Xuefei Tian, Chirag R. Parikh, Leyuan Xu, Jonathan Barasch, Shuta Ishibe, Lloyd G. Cantley, Dennis G. Moledina
Figure 1
Urinary LIF is associated with recovery after AIN.
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(A ) Volcano plot of proteins associated with recovery, controlled for age, sex, and urine creatinine (2-tailed t test with multiple testing correction). (B and C ) Predictive models for recovery in AIN in test (B ) and validation (C ) cohorts (ROC analysis). (D ) LIF measured by immunoassay. (E ) Cells expressing LIF. (F ) Factors downstream of LIFR signaling, including SOCS3, have higher expression in neighbors of LIF+ cells compared with nonneighbors. (G ) Among injured proximal tubule cells, SOCS3 expression is higher when closer to LIF+ cells (Wilcoxon rank-sum test). (H and I ) Immunofluorescence quantification of percentage of p-STAT3+ nuclei (within tubular and interstitial compartments) and SOCS3 expression. Fluorescence intensity in arbitrary units, mean ± SEM (2-way ANOVA [H ] and 1-way ANOVA [I ]). Two nephrectomy healthy controls (regions of interest [ROI] = 15), 2 AIN low-urine-LIF patients with nonrecovery (ROI = 15), and 3 AIN high-urine-LIF patients with recovery (ROI = 30).
